• icondeanrnd@iul.ac.in, diriirc@iul.ac.in

About Integral Referral Laboratory for Biological Analysis and Research

About Integral Referral Laboratory for Biological Analysis and Research

The Integral Referral Laboratory for Biological Analysis and Research (IRL-BAR) is a state-of-the-art facility for conducting cutting edge interdisciplinary research. The facility is equipped with high-end instruments including, confocal microscope, flow cytometer, real-time PCR machine, and chemi- documentation system. IRL-BAR offers gene expression analysis, cell cycle analysis, cell surface marker analysis, SNP analysis, copy number evaluation, 3D-imaging, FRET analysis, time-lapse microscopy, cellular uptake assay, toxicity assays, and others. These services are available to researchers, university students, academic institutions, medical establishments, national and international research institutes, as well as industries. We are committed to provide quality and reliable data, in both hard copy and electronic formats.

B.1 LEICA STELLARIS 5 CONFOCAL MICROSCOPE:

STELLARIS 5 stands as a revolutionary core system, transcending the boundaries of traditional confocal microscopy. It ushers in a new era, offering a groundbreaking confocal system that boasts an integrated next-generation White Light Laser (WLL). Paired with our proprietary Acousto-Optical Beam Splitter (AOBS) and cutting-edge Power HyD S detectors, this system redefines the very essence of image quality and information output. The hallmark of STELLARIS 5 lies in the introduction of TauSense technology, a true game-changer. This technology introduces a range of imaging modes like TauContrast, TauGating, and TauSeparation, all founded on the principles of fluorescence lifetime, enabling functional imaging that was previously beyond reach.

STELLARIS 5 is an embodiment of perfected imaging performance, and it's all made effortlessly accessible through the user-friendly interface, ImageCompass. This interface expertly guides users through experiment setup and acquisition, making the entire process intuitive and straightforward.

At the heart of this revolutionary confocal microscope is the new generation of Power HyD detectors, an entirely optimized beam path, and the one-of-a-kind White Light Laser. This synergy results in an unparalleled imaging experience, one that opens doors to previously unattainable insights

  • 3D Imaging: Capturing the three-dimensional structure of your samples with precision.
  • Co-localization: Uncovering the intricate relationships between different components within your samples.
  • FRET (Fluorescence Resonance Energy Transfer): Examining the transfer of energy between fluorophores, enabling insights into molecular interactions.
  • Fluorescence Recovery After Photobleaching (FRAP): Aiding in the study of dynamic processes within living cells.
  • Photoactivation: Initiating fluorescence in specific regions, a valuable tool for cellular manipulation.
  • Time-lapse Microscopy: Observing dynamic processes over time, providing insights into temporal changes.
  • Multicolor Microscopy: Exploring samples with multiple fluorescent labels simultaneously, enriching the depth of analysis.

STELLARIS 5 is not just a confocal microscope; it's a gateway to a new realm of possibilities in imaging and research.

B.2 BD FACSLyric Flow Cytometer: Elevating Research and Clinical Analysis

The BD FACSLyric flow cytometer represents a pinnacle in high-performance technology, meticulously designed to serve the dual purpose of routine analysis and clinical research. A comprehensive solution that seamlessly integrates the instrument, software, reagents, and services, BD FACSLyric stands as a beacon of precision and dependability for clinicians and scientists alike. With the capability to detect up to 10 colors and provide forward and side scatter data, this instrument offers an optimal platform for multicolor analysis. A combination of vacuum-driven fluidics, a unique sample injection tube (SIT), and an innovative cuvette design work in tandem to elevate system reliability and enhance signal resolution. The fluidics design is versatile, accommodating a wide array of sample input devices.

For the utmost convenience, the optional BD FACS™ Universal Loader allows for automated acquisition, permitting walkaway operation with samples loaded in either microtiter plates or 12 x 75-mm tube racks. All of this exceptional performance is packed into a compact footprint, ensuring that space is never a constraint.

Key Features of BD FACSLyric:

  • Cellular Uptake Studies: Uncover the intricacies of cellular uptake mechanisms.
  • Cancer Heterogeneity Analysis: Gain profound insights into the heterogeneous nature of cancer cells.
  • Detection of Apoptosis Using Annexin-PI Staining: Precisely identify apoptotic events, a critical facet in cellular research.
  • Cell Cycle Analysis: Understand the dynamics of cell division and proliferation.
  • Whole Blood Analysis: Explore the composition and characteristics of blood samples.
  • Analysis of Fluorescent Tag Expression: Assess the expression of fluorescent tags with precision.
  • Cell Surface Marker Profile Analysis: Unravel the identity of cells through their unique surface markers.
  • Cell Counting: Accurate quantification of cells, a fundamental step in many research applications.
  • Cellular Subpopulations Analysis: Discern distinct subpopulations within a cell sample, offering deeper insights into cellular diversity.

BD FACSLyric is not just an instrument; it's a gateway to a multitude of research and clinical possibilities, setting the stage for groundbreaking discoveries and advancements in the world of cellular analysis.

B. 3 ABI 7500 FAST REAL TIME PCR

Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems: Precision in Nucleic Acid Analysis

The Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems stand as an integrated and versatile pair of platforms, purpose-built for the detection and precise quantification of nucleic acid sequences. Real-time PCR, a marvel of molecular biology, seamlessly combines thermal cycling, fluorescence detection, and application-specific software. This fusion of elements allows for the meticulous measurement of PCR product accumulation, cycle by cycle, all within a single-tube homogenous reaction.

What sets real-time PCR apart is the immediate availability of quantitative results upon the conclusion of PCR, eliminating the need for gel electrophoresis, PCR product purification, or any post-PCR interventions. In comparison to traditional manual PCR quantitation methods such as Northern blotting or RNase protection assays, realtime PCR offers substantial time savings, heightened sensitivity, remarkable precision, and an extended dynamic range.

Key Advantages of the ABI 7500 Fast Real-Time PCR System:

  • Gene Expression Analysis: Delve into the intricate world of gene expression, decoding the language of life at a molecular level.
  • SNP Genotyping: Unearth genetic variations with precision, a fundamental aspect of genetics and genomics.
  • Copy Number Estimation: Accurately assess the number of copies of a specific gene or genetic sequence, providing insights into genetic stability and alterations.

The Applied Biosystems 7500 and 7500 Fast Real-Time PCR Systems stand as the epitome of excellence in nucleic acid analysis, providing researchers with a powerful and dependable tool to unlock the secrets of genetic information.

B.4 iBRIGHT 1500 Imaging System: Illuminating Your Research

The Invitrogen iBright Imaging Systems stand as a beacon of innovation, offering a suite of powerful features to simplify the process of imaging and analyzing western blots and gels. Armed with a high-resolution 9.1 MP camera and an array of automated capabilities, this system expedites the generation of publication-quality data without compromising precision.

At the heart of the iBright system lies a meticulously designed touchscreen interface, ensuring a seamless and user-friendly image capture experience. The system's built-in software, coupled with our standalone iBright Analysis Software, is purpose-built to streamline the image analysis workflow, simplifying the process from start to finish.

Key Advantages of the iBRIGHT CL750 System:

Imaging of EtBr-Stained Agarose Gels:

Capture sharp and clear images of agarose gels stained with ethidium bromide (EtBr), a fundamental technique in molecular biology research.

Chemiluminescence Imaging:

Illuminate the world of chemiluminescence, allowing for the visualization of proteins and other biological molecules through their light-emitting reactions.

Quantification of Bands:

Accurately measure and quantify the bands in your gels, providing vital data for research and analysis.

The iBRIGHT 1500 Imaging System is more than just a piece of equipment; it's a tool that empowers researchers to illuminate the secrets of their experiments with precision, efficiency, and convenience.

B.5 SYSTRONICS AU2702 DOUBLE BEAM TOUCH SCREEN UV-VIS SPECTROPHOTOMETER: Shedding Light on Sample Analysis

A UV-Vis spectrophotometer stands as a vital analytical instrument that quantifies the extent to which a sample absorbs discrete wavelengths of UV or visible light concerning a reference or blank sample. This characteristic is profoundly influenced by the composition of the sample, offering valuable insights into the sample's makeup and concentration. This remarkable tool finds diverse applications, from DNA and RNA analysis to monitoring bacterial growth, assessing beverages, and scrutinizing changes in protein structure, pigments, and dyes within the realm of wastewater treatment.

Our Central Instrument Facility (CIF) proudly houses the double-beam touch screen UV-VIS spectrophotometer, Model: AU2702. This state-of-the-art equipment boasts a high-resolution 1nm bandwidth and features a spacious 10.1-inch TFT-colored capacitive touch screen, setting new standards in user interface design.

The instrument's impressive in-house memory can store a vast amount of data, with the capacity to retain up to 1024M of test data and working curves. Its capabilities encompass a wide array of functions, including photometric measurement, quantitative measurement, kinetics, spectrum scanning, and specialized tests for DNA, RNA, and protein analysis, as well as multi-wavelength analysis.

The UV-Vis spectrophotometer is not merely a device but a gateway to unlocking the mysteries of sample composition and concentration, making it an indispensable tool in the realm of scientific analysis and research.

B. 6 AGILENT CARY ECLIPSE FLUORESCENCE SPECTROMETER

Fluorescence spectroscopy, known by various names such as fluorimetry or spectrofluorometry, is a transformative technique within electromagnetic spectroscopy that unveils a treasure trove of information hidden within a sample. It operates by directing a beam of light, typically ultraviolet in nature, to energize the electrons residing in specific molecules. This energy infusion triggers these molecules to emit light, often in the visible spectrum.

Within our Central Instrumentation Facility (CIF), we are proud to house the Agilent Cary Eclipse fluorescence spectrometer, a device that marries sensitivity, precision, and versatility. This instrument stands as the bedrock of routine fluorometry, with the remarkable ability to transition seamlessly between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Inherent adaptability is further extended through a diverse range of accessories, including options for temperature control, handling solid samples, automatic polarizers, and a microplate reader for expeditious sample analysis.

Within our Central Instrumentation Facility (CIF), we are proud to house the Agilent Cary Eclipse fluorescence spectrometer, a device that marries sensitivity, precision, and versatility. This instrument stands as the bedrock of routine fluorometry, with the remarkable ability to transition seamlessly between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Inherent adaptability is further extended through a diverse range of accessories, including options for temperature control, handling solid samples, automatic polarizers, and a microplate reader for expeditious sample analysis.

The significance of the Cary Eclipse fluorescence spectrometer extends across a broad spectrum of scientific disciplines, including life sciences, biotechnology, and various branches of biochemical, medical, and chemical research. It excels in the analysis of organic compounds, possessing not only exceptional sensitivity but also intrinsic safeguards against bleaching, an invaluable feature when handling photosensitive samples. Rapid data collection opens doors to kinetic measurements, while the convenience of fiber optics allows for remote sample analysis, offering unparalleled flexibility in experimental design.

In essence, the Agilent Cary Eclipse fluorescence spectrometer is more than a piece of equipment; it is a beacon of discovery and a cornerstone of precision in the realm of scientific exploration, guiding researchers towards a deeper understanding of the microscopic world.